Sperm-specific glyceraldehyde 3-phosphate dehydrogenase (GAPDS) activities, the intracellular adenosine 5′-triphosphate (ATP) levels, 3′-5′- cyclic adenosine monophosphate (c AMP) levels and phosphorylation of protein kinase A (PKA) substrates in rat sperm were diminished dramatically, indicating that both glycolysis and the c AMP/PKA signaling pathway were impaired by SACH. In vitro exposure of SACH 50 µM to isolated rat epididymal sperm inhibited PTP. The aim of present work was to examine the relationship between SACH and protein tyrosine phosphorylation (PTP), which plays a critical role in regulating mammalian sperm capacitation. Its (S)-isomer, (S)-α-chlorohydrin (SACH), is known for causing infertility in animals by inhibiting glycolysis of spermatozoa. α-Chlorohydrin is a common contaminant in food. Zheng, Weiwei Yang, Bei Pi, Jingbo He, Gengsheng Qu, Weidong
(S)-α-Chlorohydrin Inhibits Protein Tyrosine Phosphorylation through Blocking Cyclic AMP - Protein Kinase A Pathway in Spermatozoa
